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Part:BBa_J61016:Design

Designed by: John Anderson   Group: Arkin Lab   (2006-08-11)


Biobrick converter for BamHI-part-EcoRI


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BglII site found at 1
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal AgeI site found at 641
    Illegal AgeI site found at 753
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

N/A



Source

PCR ca1007F/R on pSB1A2-J01064 (972 bp, EcoRI/SpeI)

PCR ca1008F/ca1009R on pSB1A2-J01064 (972 bp, EcoRI/SpeI)

PCR ca1007F/ca1009R on pSB1A2-J01064 (972 bp, EcoRI/SpeI)

Sub into pSB1A2 (EcoRI/SpeI)

Products are:

Bca1007 NotI/BamHI transfer

Bca1008 BamHI/EcoRI transfer

Bca1009 NotI/EcoRI transfer

ca1007F Forward EcoRI for 5' PspOMI NotI converter

ctctggaattcgcggccgcttctagagggccctccctatcagtgatagagattg

ca1007R Reverse SpeI for 3' BglII BamHI converter

ccgctactagtagatcttataaacgcagaaaggcccac

ca1008F Forward EcoRI for 5' BglII BamHI converter

ctctggaattcgcggccgcttctagaagatcttccctatcagtgatagagattg

ca1009R Reverse SpeI for 3' MfeI EcoRI converter

ccgctactagtcaattgtataaacgcagaaaggcccac


===References===